The rigours of bioaugumenting contaminated soils for the purpose of bioremediation can be avoided and effective remediation still achieved if suitable conditions. Strategies for inexpensive and clean in situ bioremediation of soil contaminated with diesel oil include stimulation of the. Bioremediation is a popular approach of cleaning up petroleum hydrocarbons because it is simple to maintain, applicable over large areas, costeffective and leads to the complete destruction of the contaminant 5. Dec 07, 20 bioreactorsslurry reactors or aqueous reactors are used for ex situ treatment of contaminated soil and water pumped up from a contaminated plume. Physical containment and recovery of bulk or free oil is the primary response option of choice in the united states for the cleanup of oil spills in marine and freshwater shoreline environments. Used motor oil is similar to unused oil, except that it contains additional chemicals that are produced or build up in the oil, when it is used as an engine lubricant at high temperatures and pressures, inside an engine as it runs dorsey et. The test set up was done in four separated treatments. There was a general increase in microbial count for all the treatments with.
Pdf biodegradation of used engine oil by bacteria isolated. Lower costs compared to treating the soil at the original site or at an intermediate. Bioremediation of soil contaminated with used motor oil in a. The biodegradation of used lubricating oil in soil throughout the period of. The oil was rubia ld 10w30, manufactured by total for diesel motors. The first method simply moves the contamination elsewhere and may create significant risks in the excavation, handling, and transport of.
Pdf bioremediation of soil contaminated with used motor oil in a. A study was carried out on biodegradation of soil contaminated with used motor oil in aerobic fixed bed bioreactors. Feb 16, 2016 this work was aimed at evaluating the relative merits of bioaugmentation, biostimulation and surfactantenhanced bioavailability of a desert soil contaminated by crude oil through biopile treatment. Frankenberger b, a department of soil science, federal university of rio grande do sul, porto alegre rs 91540000, brazil b department of environmental sciences084.
The biodegradation potential of five bacteria isolated from used engine oil contaminated soil was investigated in this study. A mass of 100g of contaminated soil was added to all four treatments with. The degradation of used motor oil in soil as affected by plant treatment phytoremediation was assessed in a growth chamber study. Relative bioremediation of used engine oil contaminated soil from an industrialised area by various microbes article pdf available in research. The dominant microorganisms present in soils, contaminated with hydrocarbon fractions in engine oils used and unused at various automobile workshops in. Bacteria and fungi were enumerated using serial dilutions and pour plate method. This is due to the fact that oil tends to force the soil particles to stick together, thereby decreasing the pores. Bioremediation of spent engine oil contaminated soil by. The effect of crude oil on the soil depends on the size, quantity and grade of oil spilled. These results were obtained from field demonstration involving the bioremediation of oil contaminated soil in kuwait 2160 m 3, using three different methods, namely landfarming, composting piles 480 m 3, and static bioventing piles 240 m 3. Ex situ bioremediation approaches include use of bioreactors, landfarming, and biopiles. Bioremediation of soils contaminated by hydrocarbons is an established method these days and has been put in practice in several ways such as insitu or exsitu technologies 1,2,3,4,5. The total petroleum hydrocarbon tph concentration in the contaminated site was between 5000 to 50 000 mg kg soil, and in two locations, the tph concentration reached about 000 mgkg soil. Bioreactorsslurry reactors or aqueous reactors are used for ex situ treatment of contaminated soil and water pumped up from a contaminated plume.
Investigation into the effect of initial concentration of used engine oil, in soil, for earthworm inoculated samples showed that the biodegradation rate of used engine oil contaminant increased with increasing initial concentration of used. Alkali hydrolysable nitrogen alkaline solution diffusion method is for alkali hydrolysable nitrogen 9. Materials and methods slight modification of the method presented in agarry et al. The bacteria were inoculated into used oil supplemented with mineral salt medium and incubated at 37 o c for 25. Crude oil spillage decreases the porosity of the soil lolomari 1979. Six treatments, labeled tr1 to tr6, were investigated in an experimental rig. Used engine oil consists of petroleum ether or benzine,gasoline, naptha.
All the microorganisms used in this study had the abilities to. Bioremediation of engineoil contaminated soil using local residual. The objective of this work is to characterize the organisms indigenous to the soil samples contaminated with engine oil and to apply the. In physicochemical treatment incineration, thermal desorbtion, coker, cement kiln and solvent extraction are used but they have some disadvantages. At each sampling event two new holes were drilled with a manual. Phytoremediation of soil contaminated with used motor oil. Case study of bioremediation of soil contaminated by diesel oil. Bioremediation of crude oil polluted soil using animal waste. Frankenberger b, a department of soil science, federal university of rio grande do sul, porto alegre rs 91540000, brazil. Pdf relative bioremediation of used engine oil contaminated.
Biodegradation of used motor oil in soil using organic. Used engine oil consists of petroleum ether or benzine,gasoline, naptha, mineral spirits, kerosene, fuel oil, lubricating oil, paraffin wax, asphalt or tar. Investigation into the effect of initial concentration of used engine oil, in soil, for earthworm inoculated samples showed that the biodegradation rate of used engine oil contaminant increased with increasing initial. Higgins and burns 1979 reported that in oilcontaminated soil. In the in vitro assay, mycelium of a seven day old culture of penicillium sp. Bioremediation of spent lubricating oil contaminated soil by amending with 10% lime fertilizer was studied for a period of 56 days.
Used motor oil is the browntoblack oily liquid removed from a motor vehicle, when the oil. As a result of this accident, approximately 0 m 3 of diesel oil was released to the environment and freely flowed as a small stream, contaminating the loamy soil in that site. Pdf bioremediation of soil contaminated with used motor. Bioremediation of oil contaminated soil in kuwait springerlink. Studies on mycoremediation of used engine oil contaminated soil. In view of the pollution of the underground water, economic loss, decreased in agricultural productivity of soil, poor animal and human health caused by contamination of soil with used and unused motor oil, a study was conducted for bioremediation of.
A comprehensive guide of remediation technologies for oil. Bioremediation can be an alternative green technology for remediation of such hydrocarboncontaminated soil. Commonly used physical methods include booming and skimming, manual removal wiping, mechanical removal, water flushing, sediment relocation, and tilling. Action plan for soils contaminated with crude oil located in an oil production field in texas. The discharged oil formed over 300 oil lakes covering land areas in excess of 49 km 2, besides aerial fallout deposits which covered kuwait desert soil. The crude oil contaminated soil was generated from storage tank bottom materials which had been removed from several oil recovery storage tank batteries located within the oil field storage facility. Rcwf was equivalent to fert but retained more moisture. Comparative studies on the bioremediation of used engine oil. Comparative studies on the bioremediation of used engine. The density of oil contamination ranged from oil lakes, heavy contaminated soil 10% oil, medium and light. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation fatima m. The contaminated material is fed into a burner where takes place the volatilization and oxidation of organic compounds at temperatures between 870c and 1200. Bioremediation of hydrocarbon contaminated soil using selected. Bioremediation of crude oil contaminated soil by petroleum.
Bioremediation of contaminated soils linkedin slideshare. Introduction to enhanced oil recovery eor processes and bioremediation of oil contaminated sites 212 previous work 11 using marine filamentous bacteria reported rates of biodegradation in the range of 7. The objective of this work is to characterize the organisms indigenous to the soil samples contaminated with engine oil. Bioremediation method is considered to be more economical and safe method for the treatment. Biodegradation of used engine oil by bacteria isolated. Therefore, this study was conducted to suggest a new technique of treatment to clean up the biodiesel and diesel contaminated soil by pseudomonas putida. Optimization of nutrients requirements for bioremediation. Soil contaminated with petroleum hydrocarbons are remediated using a diverse set of physicochemical and biological biodegradation and bioremediation treatment. Effect of earthworm inoculation on the bioremediation of used. Bioremediation of petroleum oil contaminated soil and water. Spill simulations with biodiesel, diesel and their blends in sandy gravel soil were performed. Insitu bioremediation of oil contaminated soil practical.
Isolation and characterization of microorganism from oil. The remediation techniques used to improve the crude oil contaminated soil include bioremediation yan et al. The four treatment samples were tilled twice a week and watered with 50 ml of distilled water weekly. Jul 29, 2004 bioremediation of spent engine oil contaminated soil by using fungus, penicillium sp. There was a general increase in microbial count for all the. A general essay on bioremediation of contaminated soil msu.
Relative bioremediation of used engine oil contaminated soil from an industrialised area by various microbes article pdf available in research journal of pharmacy and technology 121. The impact of cow dung on the ability of eleusine indica to remediate crude oil artificially added to a particular soil was studied by 27. Moreover, the aim was 3 to study the potential for natural attenuation and the indigenous bacteria in soil, and possible adaptation to degrade diesel hydrocarbons. Researchers estimate that this cost could be reduced further in largescale applications and if containment liners are not required. In order to control the concentration and homogeneity of the contamination, the initial soil was artificially contaminated with unused engine oil to 7,500 mg kg. Pdf bioremediation of engineoil contaminated soil using. Petroleum contaminated soil the oil was dissolved in petroleum ether, and the soil was added to the soil to dry the 7 d, during which the petroleum ether completely volatilizedwas, that was, the soil containing 2% of the oil. The fungal strains were isolated from used motor oil contaminated soil samples using standard pour plate method. Environmental protection agency national risk management research laboratory. The phc concentrations were followed from june to november 2015. The first method simply moves the contamination elsewhere and may create significant risks in the ex. The autoclaved malt extract agar was then cooled down to. In the use of a bioreactor, contaminated soil is mixed with water and nutrients and the mixture is agitated by a mechanical bioreactor to stimulate.
Used engine oil can be considered as one of the source responsible for polluting the soil with hydrocarbons. Comparative bioremediation of soils contaminated with. Soil and surface water contamination by used lubricating oil is a common occurrence in most developing countries. In insitu bioremediation of oil contaminated soil by bioaugmentation oil degrading bacteria are injected into the soil matrix and the conditions for degradation are optimized by adding nutrients and electronacceptors and a detergent to desorb the hydrocarbons. Bioremediation of diesel oil contaminated soil and water. Bioremediation of engineoil contaminated soil using local residual organic matter. This has been shown to have harmful effects on the environment and human beings at large. Bergey dh, buchanan re, gibbons ne 1974 bergeys manual of. Effect of earthworm inoculation on the bioremediation of. Bioremediation of engineoil contaminated soil using local. Similarly, other work12 using pseudomonas showed that the highest oil degradation rate was from 1.
Used motor oil is the browntoblack oily liquid removed from a motor vehicle, when the oil is changed. The conventional techniques used for remediation have been to dig up contaminated soil and remove it to a landfill, or to cap and contain the contaminated areas of a site. Ex situ bioremediation, in which contaminated soil is excavated and treated elsewhere, is an alternative. However, the effectiveness of soil bioremediation, both technical and economic, has been much debated and is still the subject of numerous research. The total area contaminated by the diesel oil was about 7000 m 2. Bioremediation of used motor oil contaminated soil using elephant and horse dung as stimulants. Pdf bioremediation of used motor oil contaminated soil. During the gulf crisis, the state of kuwait was subjected to environmental hazards during oil well fires. They found that the quantities of fertilizer npk i. Optimization of nutrients requirements for bioremediation of. Bioremediation of engine oil contaminated site semantic scholar. This study originates from an accidental event of diesel oil contamination due to an overflow of a fuel storage tank.
The diesel oil was concentrated especially in three ponds along the stream. Microbial treatment of soil contaminated with spent engine oil. Pdf bioremediation of used motor oil contaminated soil using. The material used for the experiment was oil based mud obm it is contaminated soil with crude oil, the soil samples come from cabinda. The density of oil contamination ranged from oil lakes, heavy contaminated soil 10% oil, medium and light contaminated soil oil.
Bioremediation in reactors involves the processing of contaminated solid material soil, sediment, sludge or water through an engineered containment system. Crude oil contaminated soil from isaka mangrove in okirika local government area of rivers state was treated with three different organic wastes goat manure, poultry droppings and cow dung, for a period of 28 days. Biodegradation of used motor oil in soil using organic waste. Pdf a study was carried out on biodegradation of soil contaminated with used motor oil in aerobic fixed bed bioreactors. Therefore, the two main approaches to oil spill bioremediation are. Suidan, university of cincinnati cincinnati, oh 45221 u. The effect of earthworm eudrilus eugeniae on the bioremediation of used engine oil contaminated soil, amended with poultry manure, was investigated.
The impact of cow dung on the ability of eleusine indica to remediate crude. Comparative bioremediation of soils contaminated with diesel. Bioremediation of used motor oil contaminated soil using elephant and horse dung as stimulants was conducted by 25. Case study of bioremediation of soil contaminated by. This study investigated the ability of penicillium sp. Bioremediation of crude oil contaminated desert soil. Bioremediation can be an alternative green technology for remediation of such hydrocarbon contaminated soil. Used motor oil is a common and toxic environmental contaminant, and technologies for its remediation vary in success. Therefore, the two main approaches to oilspill bioremediation are. The results show that the desert soil required bioaugmentation and biostimulation for bioremediation of crude oil. Introduction crude oil is the largest and most commonly used source of energy in the world, major portions of it are used as transportations fuel such as kerosene, gasoline and diesel. Results indicate that manrf was the only amendment more effective than fert for phc reduction, while emitting the least co2 overall. Bioremediation of soil contaminated with spent motor oil. A general essay on bioremediation of contaminated soil.